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KMID : 0903619890300020145
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Journal of the Korean Society for Horticultural Science
1989 Volume.30 No. 2 p.145 ~ p.156
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In Vitro Pollination , Ovule Culture of Hibiscus syriacus and Differentiation of Adventitious Bud and Somatic Embryo from Callus
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Abstract
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In vitro pollination and ovule culture after intra-and interspeeific crossing between Hibscus syriacus and H. rosa-sinensis on the field were conducted in the present experiment to evaluate possibility of improving H. syriacus L. Histological observations were also made on the development of adventitious buds and somatic embryos from calli which were obtained by culturing immature embryos. In vitro cross-pollination on ovularies between H. syriacus and H. rosasinensis brought about only the growth of ovularies, whereas ovules pollinated in vitro between these two species died out after 8 weeks in culture. The growth of ovularies and ovules by in vitro pollination was better on Murashige-Skoog medium than on White or Nitsch media, 3% sucrose being most effective. In case of intraspecific hybridization, initiation of embryo formation was observed 15 days after crossing and ovule culture performed 20 days after pollination resulted in 100 plant development. In H. Syriacus × H. rosa-sinensis crossing, no plant was developed from ovule culture. Somatic embryos and adventitious buds were initiated from shoot-forming callus. Vascular cylinders which were consisted of short tracheids, and tracheid nest were observed and so it was presumed that roots were differentiated from these tissues.
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